Preadipocyte promotes cell invasion of prostate cancer by CAMKK2
10.3760/cma.j.cn431274-20190808-00936
- VernacularTitle:CAMKK2参与前脂肪细胞对前列腺癌侵袭力影响的功能研究
- Author:
Jia SUI
1
;
Wenke SONG
;
Fan ZHANG
;
Yunrong LI
;
Xuejian ZHAN
;
Hao FU
Author Information
1. 南华大学附属南华医院泌尿外科，衡阳 421002
- From:
Journal of Chinese Physician
2020;22(6):834-837,842
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Calcium/calmodulin-dependent kinase kinase (CAMKK2) is activated in prostate cancer (PCa). The aim of our study is to identify the effects of preadipocyte and CAMKK2 on the PCa cell invasion.Methods:The coculture system between PCa cells and preadipocyte cells (3T3L1) was established and then Transwell assay was used to determine the invasive effect after coculture. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to investigate the CAMKK2 expression in the coculture system.Results:The 3T3L1 cells promoted the invasion of PCa cells (DU145 and 22RV1; P<0.01). Meanwhile, the 3T3L1 cells can increased expression of CAMKK2 in PCa cells ( P<0.01). CAMKK2 reversed the invasive effects of preadipocyte cells on the PCa cells. Conclusions:Our study demonstrates that the preadipocyte may promote the PCa cell invasion possibly by mediating the CAMKK2 expression.
