Network correlation of circRNA-miRNA and regulatory mechanisms of female major depressive disorder
10.3760/cma.j.cn371468-20201007-01770
- VernacularTitle:女性重度抑郁障碍circRNA-miRNA网络相关性及调控机制分析
- Author:
Yunmiao YU
1
;
Tianyi BU
;
Xiaohui QIU
;
Xiuxian YANG
;
Zhengxue QIAO
;
Erying ZHAO
;
Jiawei ZHOU
;
Wenxin ZHANG
;
Ye SUN
;
Yanjie YANG
Author Information
1. 哈尔滨医科大学公共卫生学院医学心理学教研室 150081
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2020;29(11):1007-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain differential expression profiles of circRNAs and miRNAs in peripheral blood of female patients with major depressive disorder based on high-throughput sequencing technology, and then construct an interaction network.Based on the outcome, it made a further exploration of the possible occurrence and development mechanisms of female major depressive disorder through functional annotation and pathway enrichment analysis.Methods:According to the ceRNA theory, circRNA-miRNA network was constructed by TargetScan software via predicting the binding sites.Subsequently, the GO functional enrichment analysis was performed, and KEGG pathway enrichment analysis were utlized to illustrate the target genes of co-expressed miRNAs.Thereby, the key genes related to major depressive disorder could be screened out.Results:A total of 724 differential circRNAs and 26 differential miRNAs were detected in female patients with major depressive disorder.And hsa_circ_0086092 and hsa-miR-146a-3p were the most co-expressed.Go functional annotations pointed out that it involved the regulation of nucleobase-containing compound metabolic process, regulation of RNA splicing, regulation of cell communication, amino acid transfer, regulation of RNA metabolic process, regulation of signaling and other biological processes.KEGG pathway analysis showed that target genes were mainly enriched in neurotrophin signaling pathway, Rap1 signaling pathway, FoxO signaling pathway, AMPK signaling pathway, cocaine addiction, mTOR signaling pathway, Jak-STAT signaling pathway, cAMP signaling pathway, etcetera.Among the predicted target genes, BDNF, FGF2, MAPK14, GRIN2A, GRIN2B, GRM2 and PDE4 have the highest correlation with major depressive disorder.Conclusion:Hsa_circ_0086092 may be involved in the occurrence and development of female major depressive disorder through interaction with hsa-miR-146a-3p.
