Bioinformatics analysis of peripheral blood circRNAs in female patients with major depressive disorder
10.3760/cma.j.cn371468-20200106-00991
- VernacularTitle:女性重度抑郁障碍患者外周血环状RNA的生物信息分析
- Author:
Wenxin ZHANG
1
;
Yunmiao YU
;
Tianyi BU
;
Xiaohui QIU
;
Xiuxian YANG
;
Zhengxue QIAO
;
Mingzhe ZHAO
;
Yanjie YANG
Author Information
1. 哈尔滨医科大学公共卫生学院医学心理学教研室，哈尔滨 150081
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2020;29(8):725-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen the circRNAs with differential expression between female patients with major depressive disorder and healthy women, and to explore the circRNAs that might be associated with depressive disorder through bioinformatics analysis.Methods:Using high-throughput sequencing screen differentially expressed circRNAs of female major depressive disorder patients, |log 2FC|≥1 and FDR<0.05 were used to determine whether circRNA had a difference in expression.According to the miRNA sponges function of circRNA, the online databases were used to predict miRNAs which may be targeted by circRNAs, and miRNAs related target genes were also predicted by the five most different circRNA.GO and KEGG pathway enrichment analysis were used to predict biological processes for the target genes and signaling pathways.Based on the results of high-throughput sequencing, biological processes and signaling pathways related to depression, circRNAs related to depression were screened. Results:Thirteen targeted miRNAs were predicted by the five most different circRNAs(hsa_circ_0020959, hsa_circ_0005959, hsa_circ_0033064, hsa_circ_0006862, hsa_circ_0027732), and multiple biological processes and signaling pathways related to depressive disorders were predicted by the target genes, such as glucocorticoid receptor signaling pathway, interleukin-7 response, nervous system development, Wnt signaling pathway, FoxO signaling pathway, thyroid hormone signaling pathway, neurotrophin signaling pathway, and so on.Conclusion:All the 5 circRNAs enriched biological processes or signaling pathways related to depressive disorder, among which hsa_circ_0005959, hsa_circ_0033064, hsa_circ_0006862 and hsa_circ_0027732 may be more closely related to female major depressive disorder.
