Expression of triggering receptor expressed on myeloid cells in periventricular leukomalacia of neonatal rat model
10.3760/cma.j.cn371468-20191213-00912
- VernacularTitle:髓样细胞触发受体在新生大鼠脑室周围白质软化模型中的表达
- Author:
Dayu WANG
1
;
Xiaoyan YE
;
Yan XU
;
Jun WANG
Author Information
1. 徐州医科大学附属医院儿科 221000
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2020;29(2):109-113
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of triggering receptor expressed on myeloid cells(TREMs)in periventricular leukomalacia(PVL) of the neonatal rat model.Methods:Thirty-two 3-day-old neonatal rats were double-blinded randomly divided into Sham group and Model group.The PVL rat model was established by ligating right carotid artery and oxygen deprivation.Hematoxylin-eosin (HE) was adopted to compare pathological changes of brain tissue between the two groups, and immunofluorescence was adopted to detect the expression of myline basic protein (MBP) in the right hemisphere of the two groups.Western blot was performed to detect the expression of TREM1 and TREM2 in the right hemisphere of the two groups.Results:The results of HE staining showed that the brain tissues of Model group were significantly damaged compared with that of Sham group, and the mean fluorescence intensity of MBP in Model group(26.629±2.317) was significantly lower than that in Sham group(33.579±2.824), with statistically significant differences( t=9.124, P<0.05). The expression of TREM1 in Model group(0.789 ±0.120) was higher than that in Sham group(0.567±0.093), with statistically significant differences( t=-3.891, P<0.05). The expression of TREM2 in Model group(0.544±0.133) was lower than that in Sham group(0.791±0.118), with statistically significant differences( t=3.667, P<0.05). Conclusion:The expressions of TREM1 and TREM2 in the neonatal rat model of PVL change abnormally, suggesting that TREMs may be involved in the pathological process of preterm white matter injury.
