Changes and clinical significance of intestinal Bacteroides in obese children
10.3760/cma.j.issn.1008-6706.2020.18.015
- VernacularTitle:肥胖儿童肠道拟杆菌属的变化及意义
- Author:
Wanying ZHU
1
;
Li ZHANG
;
Kebin LU
;
Min ZHAO
;
Yanping YUAN
Author Information
1. 山东省单县中心医院 济宁医学院附属湖西医院儿科 274300
- From:
Chinese Journal of Primary Medicine and Pharmacy
2020;27(18):2238-2244
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the diversity of Bacteroides in simple obesity children aged 7-14 and the difference from that of normal individuals.And to explore the influence of children's simple obesity on intestinal advantage bacterium of the genus.Methods:We have collected fecal samples of 30 cases with simple obesity aged 7 to 14 years old and 30 healthy children in the Central Hospital of Shan County.All bacterial DNAs were extracted from the feces, and then Bacteroides 16s DNA specific primers was used in PCR amplification, separated by DGGE, the intestinal Bacteroides molecular fingerprints of obese children and healthy children were obtained, and the diversity and similarity were analyzed.At the same time, the weight, height, BMI, blood pressure, blood lipid, leptin, plasma adiponectin in the obesity and normal children were measured, the relationship between the obese children and the advantage of intestinal bacteria genera and its clinical significance were investigated.Results:The BMI[(25.71±3.28)kg/m 2], SBP[(121±12.4)mmHg], TG[(1.15±0.40)mmol/L], TC[(4.20±0.73)mmol/L], LEP(18.94±1.68) in the obesity group were higher than those in the normal group [BMI(17.38±3.54)kg/m 2, SBP(104±7.3)mmHg, TG(0.87±0.31)mmol/L, TC(3.37±0.86)mmol/L, LEP(11.29±2.41)](all P<0.05). The levels of HDL[(1.01±0.28)mmol/L] and ADP(4.10±2.32) in the obesity group were lower than those in the normal group [HDL(1.40±0.26)mmol/L, ADP(5.84±3.42)]( P<0.05). There were no statistically significant differences in age, DBP and LDL levels between the two groups (all P>0.05). The stripe number of Bacteroides DGGE profiles (9.33±1.37) and H index(2.07±0.16) in the obesity group were lower than those in the normal group [(17.33±2.80), (2.67±0.20)]. Cluster analysis results showed that the similarities of obese individuals in the group and compared with normal group were reduced.The S and H index of DGGE profiles in Bacteroides were negatively correlated with BMI and LEP ( P<0.01), and positively correlated with ADP( P<0.05). S, H index of the normal group were negatively correlated with LEP ( P<0.05), and had no correlation with BMI, ADP( P>0.05). S and H index of the obesity group were negatively correlated with BMI and LEP, and had no correlation with ADP ( P>0.05). Conclusion:Human intestinal flora of Bacteroides changes is related to childhood obesity, the diversity and similarity of obese children's intestinal Bacteroides species are decreased.The diversity of obesity children's Bacteroides is associated with BMI, ADP and LEP.
