Establishment and application of detection method for minimum inhibitory concentration of Yersinia pestis
10.3760/cma.j.cn231583-20190621-00179
- VernacularTitle:鼠疫耶尔森菌最低抑菌浓度测定方法的建立与应用
- Author:
Jian HE
1
;
Xiaoyan YANG
;
Youquan XIN
;
Juan JIN
;
Sheng LI
;
Qi ZHANG
;
Jixiang BAI
;
Hailian WU
;
Yong JIN
;
Xuefei ZHANG
;
Ruixia DAI
;
Baiqing WEI
Author Information
1. 青海省地方病预防控制所鼠疫菌专业实验室，西宁 811602
- From:
Chinese Journal of Endemiology
2020;39(11):842-846
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the minimum inhibitory concentration (MIC) detection method of Yersinia pestis by determining MIC of 11 kinds of antibiotics against Yersinia pestis, to master the inhibition range of common antibiotics on Yersinia pestis, and provide baseline data for clinical treatment of plague. Methods:According to Clinical Labor Standard Institution (CLSI), the agar plate dilution method was used to determine the MIC of 11 kinds of antibiotics against 118 strains of Yersinia pestis, including ofloxacin, ciprofloxacin, trimethoprim-sulfamethoxazole, kanamycin, streptomycin, ceftriaxone, ampicillin, chloramphenicol, spectinomycin, cefuroxime and tetracycline. MIC 50 and MIC 90 (the minimum concentration of drug which could inhibit 50% and 90% of bacterial growth) were calculated. The consistency was observed by comparing the results with those of the disk diffusion method. One hundred and eighteen strains of Yersinia pestis were isolated from natural plague foci of Qinghai Province and preserved by Qinghai Institute for Endemic Disease Prevention and Control. Results:Among 118 strains of Yersinia pestis tested, no single or multiple strains of Yersinia pestis resistant to 11 kinds of antibiotics were found, which was consistent with the results of the disk diffusion method. The MIC 50 and MIC 90 of 11 kinds of antibiotics against 118 strains of Yersinia pestis were obtained. Conclusions:The MIC detection method of Yersinia pestis is successfully established. This method can be used to measure the MIC of antibiotics against Yersinia pestis in high throughput and evaluate the sensitivity of Yersinia pestis to antibiotics. It is an efficient, economical and practical experimental method.
