Molecular diagnosis and characterization analysis of the first case of human Brucella suis infection in Jiangsu Province
10.3760/cma.j.cn231583-20191113-00317
- VernacularTitle:江苏省首例人感染猪种布鲁菌的分子诊断及特征分析
- Author:
Zhongming TAN
1
;
Xiubin WANG
;
Weizhong ZHOU
;
Chen DONG
;
Lu ZHOU
;
Jie HONG
;
Huimin QIAN
;
Jianli HU
;
Changjun BAO
Author Information
1. 国家卫生健康委员会肠道病原微生物重点实验室 江苏省疾病预防控制中心，南京 210009
- From:
Chinese Journal of Endemiology
2020;39(11):781-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify human infection with Brucella suis, analyze its biological and molecular characteristics, and to provide basis for prevention and control of brucellosis. Methods:Brucella suis strains were isolated from the body of the first case of human Brucella suis infection in Jiangsu Province. Serum agglutination test was used for serotyping. The specific gene bcsp-31 of Brucella was detected by PCR. AMOS-PCR was used to identify IS-711. The species and biotypes were identified by multiplex PCR. The wboA gene products were sequenced and phylogenetic tree was constructed. Multilocus sequence analysis (MLSA) was used for molecular typing, and cluster analysis was performed with reference strains. Results:The strain was confirmed to be Brucella suis biotype 3 by serum agglutination test and PCR. After sequencing the wboA gene, cluster analysis of the reference sequence showed that the wboA gene was closest to the biotype 3 strain Brucella suis str. 686 (CP007719). MLSA was typed into ST17(1-6-4-1-5-3-5-2-4). Conclusions:Brucella suis biotype 3 is reported in Jiangsu Province for the first time. The MLSA type is ST17. In the future, the prevention and control of human brucellosis should be carried out. We should actively cooperate with the animal husbandry and veterinary department to increase the quarantine, immunization and other control measures.
