Establishment and evaluation of a high-throughput and rapid method for detection of fluorine content in water based on microplate reader
10.3760/cma.j.cn231583-20191031-00302
- VernacularTitle:基于酶标仪的高通量快速水氟含量检测方法的建立与评价
- Author:
Liaowei WU
1
;
Simeng HUO
;
Yanru CHU
;
Wei HUANG
;
Jian WANG
;
Yanmei YANG
;
Yanhui GAO
;
Wei WANG
Author Information
1. 哈尔滨医科大学中国疾病预防控制中心地方病控制中心地氟病防治研究所 国家卫生健康委员会病因流行病学重点实验室 150081
- From:
Chinese Journal of Endemiology
2020;39(9):692-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study and establish a high-throughput and rapid detection method for fluorine ion content in water based on 96-well plate and microplate reader, and to evaluate its performance.Methods:According to the principle of blue ternary complex formed by reaction of fluorine ion with fluorine reagent and lanthanum nitrate, the absorbance of the complex at the wavelength of 650 nm was proportional to the concentration of fluoride ion, and the content of fluoride ion in water samples was quantitatively determined by microplate reader. The accuracy, precision and detection limit of the method were evaluated by the standard recovery rate method and the reference material method.Results:The linear correlation coefficient of the standard curve of this method was > 0.999; the standard recovery rates of different concentrations were 99.00% - 103.33% in the accuracy experiment of this method, and the average recovery rate was 101.08%. In the standard material method, the measured values are all within the calibration range of the standard solution. The relative standard deviation of water samples with different concentrations was ≤10% in the precision experiment and the detection limit was 0.06 mg/L.Conclusion:The method has good accuracy and precision, low detection limit and small sample consumption, and this method can greatly improve the detection efficiency.
