MLVA genotyping characteristics of 13 strains of Brucella isolated from non-brucellosis epidemic area Lianyungang City of Jiangsu Province
10.3760/cma.j.cn231583-20190906-00246
- VernacularTitle:非布鲁菌病疫区江苏省连云港市13株布鲁菌分离株的MLVA基因分型特征分析
- Author:
Danting JIN
1
;
Chunlei ZUO
;
Xinzhe WU
;
Hongshu LIU
;
Yong ZHAO
;
Zhongming TAN
;
Haifeng MAO
Author Information
1. 江苏省连云港市第一人民医院检验科 222002
- From:
Chinese Journal of Endemiology
2020;39(8):547-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the genotyping characteristics of Brucella strains isolated from Lianyungang City (non-brucellosis epidemic area) of Jiangsu Province. Methods:Preliminary identification of 13 suspected strains of Brucella isolated from blood culture in Clinical Microbiology Laboratory of the First People's Hospital of Lianyungang City in 2018 was conducted; at the same time, the specific gene bcsp31 and insertion sequence IS-711 of Brucella were detected by quantitative real-time PCR (Real-time PCR), and the identification results were rechecked and typed. Multiple locus variable-number tandem repeat analysis (MLVA) was applied for genotyping, and the sequencing results were edited by Mega 4.0 software. Results:All the 13 strains were identified as Brucella by preliminary identification. Real-time PCR confirmed that all the 13 strains were Brucella melitensis. The results of MLVA showed that 13 strains of Brucella melitensis were divided into 12 genotypes and clustered in the "middle Mediterranean cluster". Among 13 strains of Brucella melitensis, 3 strains were biovar 1, 2 strains were biovar 2 and 8 strains were biovar 3. Conclusion:All the Brucella strains isolated from Lianyungang City are Brucella melitensis and the MLVA cluster is in the "middle Mediterranean cluster".
