Rapid determination of iodine in whole blood by direct alkali dilution ICP-MS
10.3760/cma.j.cn231583-20190802-00211
- VernacularTitle:碱直接稀释-电感耦合等离子体质谱法快速测定全血碘含量
- Author:
Yuanhua MENG
1
;
Yan GONG
;
Zhifei XU
;
Yanli GU
;
Wenwei LIU
;
Xia LING
Author Information
1. 无锡市疾病预防控制中心理化检验部，江苏无锡 214023；江南大学公共卫生研究中心，江苏无锡 214122
- From:
Chinese Journal of Endemiology
2020;39(8):606-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for rapid determination of iodine in whole blood by direct alkali dilution inductively coupled plasma mass spectrometry (ICP-MS).Methods:Totally 0.50 ml whole blood sample was collected, and 2% ammonia and 0.01% Triton X-100 solution were added to constitute a total volume of 10.0 ml. After shaking to uniformity, 1.0 μg/ml rhodium and 20% isopropanol were used as on-line internal standard solution. The flow ratio of internal standard solution to the solution to be measured was 1∶16. The sample was quantitatively determined by ICP-MS. The linear range, limit of detection (LOD), accuracy and precision of the method were evaluated.Results:Iodine in whole blood could be determined and had a good linear relationship within the range of 0-200 μg/L, with correlation coefficient ( r) > 0.999. The LOD of the method was 0.1 μg/L, the limit of quantitation (LOQ) was 0.3 μg/L, the recovery rate of iodine in whole blood was 88.5%-106.1%, and the relative standard deviation was 2.2%-4.7% ( n=7). Conclusions:A method for rapid determination of iodine in whole blood by direct alkali dilution ICP-MS is successfully established. This method is accurate, simple, rapid, and highly automatic, and it can be widely applied in determination of iodine in whole blood.
