New Approach of Bone Marrow-Derived Mesenchymal Stem Cells and Human Amniotic Epithelial Cells Applications in Accelerating Wound Healing of Irradiated Albino Rats.
- Author:
Samah S MEHANNI
1
;
Noha F IBRAHIM
;
Alyaa R HASSAN
;
Laila A RASHED
Author Information
1. Department of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt.
- Publication Type:Original Article
- Keywords:
Wound healing;
BM-MSCs;
HAECs
- MeSH:
Animals;
Biopsy;
Dermis;
Epithelial Cells;
Epithelium;
Gamma Rays;
Humans;
Inflammation;
Male;
Mesenchymal Stromal Cells;
Rats;
Skin;
Stem Cells;
Transforming Growth Factor beta1;
Wound Healing
- From:International Journal of Stem Cells
2013;6(1):45-54
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Irradiated wound healing is a highly complex and dynamic process. The latest technology making a huge difference in this process is stem cell therapy. The goal of this study was to evaluate the use of bone marrow-derived mesenchymal stem cells (BM-MSCs) or human amniotic epithelial cells (HAECs) in the healing of irradiated wounds. METHODS AND RESULTS: Forty five male albino rats were subjected to whole body 6 gray gamma radiations. One day post irradiation, full-thickness incisional wound was created in the tibial skin. The rats were randomly equally divided into three groups. The incisions of the first group (gp I) were injected intra-dermally with saline before stitching and those of both the second (gp II) and the third groups (gp III) were intradermally injected with BM-MSCs and HAECs before stitching respectively. Animals were sacrificed after the third, seventh and fourteenth days postoperative. The healing process was assessed histopathologically. CXCL-5, SDF-1 and Transforming growth factor-beta 1 (TGF-beta1) expression were also detected in biopsies from all wounds. Expression of TGF-beta1 in gp I was more than the other groups leading to severe inflammation, deficient healed dermis and delayed reepithelialization. SDF-1 expression was high in gp II while CXCL-5 expression was high in gp III causing accelerated wound healing. BM-MSCs showed a great effect on the quality of the dermis, while superiority of the epithelium and its appendages were achieved in HAECs group. CONCLUSIONS: Using BM-MSCs and HAECs could be used safely in case of irradiated wounds.
