Histological Study on Effect of Mesenchymal Stem Cell Therapy on Experimental Renal Injury Induced by Ischemia/Reperfusion in Male Albino Rat.
- Author:
Eman Mostafa SADEK
1
;
Noha Mohamed AFIFI
;
Lamiaa Ibrahim ABD ELFATTAH
;
Manal Ali ABD EL MOHSEN
Author Information
1. Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt. lamiaafattah@ymail.com
- Publication Type:Original Article
- Keywords:
Acute kidney injury;
Ischaemia reperfusion;
Mesenchymal stem cells;
Proliferating cell nuclear antigen
- MeSH:
Acute Kidney Injury;
Adult;
Animals;
Clinical Protocols;
Constriction;
Creatinine;
Humans;
Injections, Intravenous;
Kidney;
Male;
Mesenchymal Stromal Cells;
Organic Chemicals;
Proliferating Cell Nuclear Antigen;
Rats;
Urea
- From:International Journal of Stem Cells
2013;6(1):55-66
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Acute kidney injury (AKI) represents a major clinical problem with high mortality and limited treatment protocols. This study was planned to evaluate the therapeutic effectiveness of bone marrow-derived mesenchymal stem cells (BM-MSCs) in a rat model of ischemia/reperfusion (I/R) AKI. METHODS AND RESULTS: This study was carried out on thirty adult male albino rats. Animals were divided equally into three groups. Group I (control sham-operated group) (n=10), were subdivided equally into two subgroups; Ia and Ib. The experimental group (n=20) were all subjected to I/R injury by clamping both renal pedicles for 40 minutes. Half of the I/R animals did not receive MSC therapy (group II) [non-MSC treated group]. The other half of the I/R animals received single intravenous injection of PKH26 labelled BM-MSCs immediately after removal of the clamps and visual confirmation of reflow (group III) [MSC treated group]. Animals were sacrificed 24 hrs (subgroups IIa & IIIa) and 72 hrs (subgroups IIb & IIIb) after intervention. Serological measurements included serum urea and creatinine. Kidney specimens were processed for H&E, PAS and PCNA. Mean % of renal corpuscles with affected glomeruli, mean % of affected tubules, mean area % of PAS-positive reaction and mean area % of PCNA immunoreactivity were measured by histomorphometric studies and statistically compared. MSCs-treated group exhibited protection against renal injury serologically and histologically. CONCLUSIONS: Results of the present study suggest a potential reno-protective capacity of MSCs which could be of considerable therapeutic promise for cell-based management of clinical AKI.
