Study on the application of enzyme-labeled instrument in the detection of iodine in drinking water
10.3760/cma.j.issn.2095-4255.2020.01.014
- VernacularTitle:应用酶标仪测定饮用水中碘含量的方法研究
- Author:
Shudong XU
1
;
Weidong LI
;
Lisheng ZHAO
;
Tingting LIU
;
Jingjing JIANG
;
Xian XU
;
Chen YU
;
Cuicui TIAN
;
Tao ZHANG
Author Information
1. 安徽省疾病预防控制中心地方病与寄生虫病防治科，合肥 230601
- From:
Chinese Journal of Endemiology
2020;39(1):64-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an automatic colorimetric method for determination of iodine in drinking water by enzyme-labeled instrument (hereinafter referred to as this method).Methods:The water iodine was measured in the range of 0 - 10 μg/L and 0 - 100 μg/L, experiments were carried out on linear relationship, detection limit, precision and accuracy of this method. And the results were compared with the National Reference Laboratory for Iodine Deficiency Disorders recommended arsenic cerium catalytic spectrophotometry method.Results:In the range of 0 - 10 μg/L and 0 - 100 μg/L, all│ r│ > 0.999 0, the detection limits were 0.6 and 1.1 μg/L (samples were 200 and 100 μl), respectively; the relative standard deviation ( RSD) of water samples of low, medium and high iodine mass concentrations were < 3%, the recovery rates ranged from 92.5% to 108.3% and 93.2% to 108.9%, with a total average recovery of 100.0% and 100.3%, respectively. This method and arsenic cerium catalytic spectrophotometry method were used to detect 40 water samples in the range of 0 - 10 μg/L and 0 - 100 μg/L, there was no significant difference in water iodine content between the two methods ( t = 0.99, P > 0.05). Conclusion:This method has good linear curve relationship for determination of water iodine content, good precision and high accuracy, and it is suitable for wide application.
