A pyrene-based ratiometric fluorescent probe with a large Stokes shift for selective detection of hydrogen peroxide in living cells
- Author:
Qingxin CHEN
1
,
2
;
Ke CHENG
;
Wanhe WANG
;
Liu YANG
;
Yusheng XIE
;
Ling FENG
;
Jie ZHANG
;
Huatang ZHANG
;
Hongyan SUN
Author Information
1. Department of Chemistry and Center of Super-Diamond and Advanced Films, City University of Hong Kong, Hong Kong, China
2. Key Laboratory of Biochip Technology, Biotech and Health Centre, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China
- Keywords:
Hydrogen peroxide;
Fluorescent probe;
Pyrene;
Ratiometric detection;
Large Stokes shift
- From:
Journal of Pharmaceutical Analysis
2020;10(5):490-497
- CountryChina
- Language:Chinese
-
Abstract:
Hydrogen peroxide (H2O2) plays a significant role in regulating a variety of biological processes. Dys-regulation of H2O2 can lead to various diseases. Although numerous fluorescent imaging probes for H2O2 have been reported, the development of H2O2 ratiometric fluorescent probe with large Stokes shift re-mains rather limited. Such probes have shown distinct advantages, such as minimized interference from environment and improved signal-to noise ratio. In this work, we reported a new pyrene-based com-pound Py-VPB as H2O2 fluorescent probe in vitro. The probe demonstrated ratiometric detection behavior, large Stokes shift and large emission shift. In addition, the probe showed high sensitivity and selectivity towards H2O2 in vitro. Based on these excellent properties, we successfully applied Py-VPB to the visualization of exogenous and endogenous H2O2 in living cells. Cell imaging study also showed that our probe was localized in the mitochondria. We envision that the probe can provide a useful tool for unmasking the biological roles of mitochondrial H2O2 in living systems.
