Rapid detection of high-risk HPV16 and HPV18 based on microchip electrophoresis
- Author:
Zhaoxuan FAN
1
,
2
;
Xiao FENG
;
Weifei ZHANG
;
Xueji ZHANG
;
Jin-Ming LIN
Author Information
1. Research Center for Bioengineering and Sensing Technology,School of Chemistry and Biological Engineering,University of Science and Technology Beijing,Beijing,100083,PR China
2. Department of Chemistry,Beijing Key Laboratory of Microanalytical Methods and Instrumentation,MOE Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology,Tsinghua University,Beijing,100084,PR China
- Keywords:
Human papillomavirus;
DNA analysis;
Polymerase chain reaction;
Microchip electrophoresis;
Detection
- From:
Journal of Pharmaceutical Analysis
2020;10(4):329-333
- CountryChina
- Language:Chinese
-
Abstract:
Researches on detection of human papillomavirus (HPV) high-risk samples were carried out by poly-merase chain reaction (PCR) coupled with microchip electrophoresis (MCE). Herein, we introduced a simple, rapid, automated method for detecting high-risk samples HPV16 and HPV18. In this research, general primers were initially selected to obtain sufficient detectable yield by PCR to verify feasibility of MCM method for HPV detection, then type-specific primers were further used to evaluate the specificity of MCE method. The results indicated MCE method was capable of specifically detecting high-risk HPV16 and HPV18, and also enabled simultaneous detection of multiplex samples. This MCE method described here has been successfully applied to HPV detection and displayed excellent reliability demonstrating by sequencing results. The inherent capability of MCE facilitated HPV detection conducted in a small chip with automated, high throughput, massive parallelized analysis. We envision that MCE method will definitely pave a way for clinical diagnosis, and even on-site screening of cervical cancer.
