Establishment and biologicaI characters of an Sunitinib-resistant cell line of gastrointestinal stromal tumor

VernacularTitle:胃肠道间质瘤Sunitinib耐药细胞株的建立及其生物学特性的研究
Author: Cuihua WANG ¹ ; Xuetao YU ; Xinming YAO
Author Information

1. 上海市普陀区利群医院肿瘤科　200333
From: Chinese Journal of Postgraduates of Medicine 2020;43(12):1057-1059
CountryChina
Language:Chinese
Abstract: Objective:To establish the Sunitinib-resistant model GIST882-Rs.Methods:The intermittent concentration gradient doubling method was used to get Sunitinib-resistant cell line GIST 882-Rs. Changes of cell morphology were observed and compared under the opticalm icroscopy. Changes of cell cycle were assayed and compared by using flow cytometer. The growth curvature , half maximal inhibitory concentration (IC50) were calculated by using cell counting kit (CCK8) assay.Results:Significant changes occurred in the morphologic profiles, such as epithelioid shape, the increased ratio of nucleolus to cytoplasm, emerging multinucleated cel1 and increase of granule sub stances. IC50 for GIST882 and GIST 882-Rs were(127.50 ± 2.15) μmol and (23.98 ± 1.85) μmol, and there were significant differences ( P < 0.05). The resistance index of GIST882-Rs to Sunitinib was 5.30 fold of GIST882 cells. The cell cycle of GIST882 and GIST882- Rs was respectively 52.51% and 39.87% in S phase 25.22% and 32.32% in G 0/G 1 phase. Conclusions:The sensitivity of GIST 882-Rs to Sunitinib treatment is decreased significantly.