Value of sine oculis homeobox homolog 1 gene hypomethylation in the evaluation of breast cancer disease condition and prognosis
10.3760/cma.j.cn115455-20200320-00334
- VernacularTitle:同源框同源物1基因低甲基化在乳腺癌病情及预后评估中的价值研究
- Author:
Daoquan ZHANG
1
;
Chao HAN
Author Information
1. 山东省滨州市中心医院甲状腺乳腺外科 251700
- From:
Chinese Journal of Postgraduates of Medicine
2020;43(8):745-749
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the methylation status of sine oculis homeobox homolog 1 (Six1) in breast cancer and study its value in disease condition and prognosis evaluation.Methods:Methylation specific polymerase chain reaction (PCR) was used to detect the Six1 gene methylation in 80 patient with breast cancer (breast cancer group) and 50 patients with benign breast disease(benign breast disease group) in Binzhu Center Hospital from January 2015 to January 2017. The Six1 gene methylation rates in different clinicopathological factors were compared. Comparison of unmethylated and methylated Six1 gene in quality of life were made in breast cancer patients.Results:The unmethylation rate and mRNA expression of Six1 gene in breast cancer group were significantly higher than those in benign group ( P<0.05). Six1 genes in HCC1937, MCF-7, Hs 578T and Bcap-37 breast cancer cells were unmethylated. The unmethylated rate of Six1 gene were significantly higher in patients with Karnofsky score <70 scores, poorly differentiation, tumor maximum diameter ≥ 5 cm, number of lymph node metastases ≥ 9, with distant metastasis and TNM stage Ⅲ+Ⅳ class, compared with those in patients with Karnofsky score ≥ 70 scores, high and medium differentiation, maximum tumor diameter <5 cm, number of lymph node metastases<9, no distant metastases, and TNM stage Ⅰ+Ⅱ class. The patients with Six1 gene unmethylated had significantly lower quality of life compared with patients with Six1 gene methylated ( P<0.05). Conclusions:Six1 gene is hypomethylated in breast cancer and plays an important role in the evaluation of breast cancer disease condition and prognosis.
