MicroRNA-216a regulating the expression of SerpinB5 and affects the proliferation of liver cancer cells
10.3760/cma.j.cn115455-20190911-00670
- VernacularTitle:微小RNA-216a调控SerpinB5的表达对肝癌细胞增殖的影响
- Author:
Haifeng SUN
1
;
Yahuan GUO
;
Zhixiang SU
;
Xiaohui WEI
;
Baoxia LEI
;
Wenjuan CHEN
;
Yunmei WANG
;
Yanjun ZHANG
Author Information
1. 陕西省肿瘤医院肿瘤内科，西安 710061
- From:
Chinese Journal of Postgraduates of Medicine
2020;43(5):431-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differences in the expression of microRNA (miR)-216a and its target gene SerpinB5 at the tissue level, and the effects of miR-216a on the proliferation of different liver cancer cells by regulating the expression of SerpinB5.Methods:Through bioinformatics prediction and selection of miR-216a that regulated SerpinB5. the expressions in liver cancer and normal tissues were detected by real time polymerase chain reaction (PCR). The miR-216a simulacrum and inhibitor, si-Serpinb5 and pcdna3.1-Serpinb5 to HepG2 and MHCC97H (97H) were transfected with liposomes, respectively. Real time PCR and Wester-Blot were used to detect the expression of miR-216a and SerpinB5 before and after transfection, and CCK8 was used to detect the influence of both on the proliferation of liver cancer cells.Results:The expression of miR-216a in human liver cancer tissues was higher than that in adjacent tissues, and the difference was statistically significant ( P < 0.01). The expression of SerpinB5 in human liver cancer tissues was lower than that adjacent tissues, and the difference was statistically significant ( P < 0.01). In HepG2 and 97H, miR-216a inhibitor and SerpinB5 overexpression group showed down-regulated miR-216a expression, which was statistically different from the control group ( P < 0.01). The proliferation of miR-216a inhibitor and pcdna3.1-serpinb5 group was lower than the control group, with statistically significant differences ( P < 0.01). Conclusions:The high expression of SerpinB5 can inhibit the proliferation of liver cancer cells, suggesting that SerpinB5 may have an anti-oncogene effect. MiR-216a may negatively regulate the expression of SerpinB5 and affect the proliferation of HCC cells.
