Resveratrol downregulates TGF-β1 and Smad3 expression and attenuates oxidative stress in CCl4-induced kidney damage in rats
- Author:
Mohammadi SAEED
1
;
Karimi JAMSHID
;
Tavilani HEIDAR
;
Khodadadi IRAJ
;
Mohseni ROOHOLLAH
;
Hashemnia MOHAMMAD
Author Information
- Keywords: Nephrotoxicity; Transforming growth factor beta1; Resveratrol; Carbon tetrachloride
- From:Asian Pacific Journal of Tropical Biomedicine 2020;10(9):397-402
- CountryChina
- Language:Chinese
- Abstract: Objective: To evaluate the effect of resveratrol against CCl4-induced nephrotoxicity. Methods: Forty-two male Wistar rats were divided into seven groups randomly. After six weeks, kidney weight, body weight, blood urea, serum creatinine, oxidative stress markers, and gene expression of renal transforming growth factor-beta1 (TGF-β1), TGF-β receptor type 1 (TGF-βR1) and Smad3 were determined. In addition, the protein level of TGF-β1 in the tissue lysate was measured. Results: Resveratrol had a protective role in renal tissue by the improvement of antioxidant balance and reduction of renal parameters such as creatinine and urea (P<0.001). In addition, the renal mRNA level of TGF-β1, TGF-βR1, Smad3, as well as the protein level of TGF-β1 were decreased in rats treated with resveratrol (P<0.001), compared to the CCl4 group. Conclusions: Overall, resveratrol shows a protective effect against nephrotoxicity in CCl4 treated rats by reducing oxidative stress status and modulating the TGF-β signaling.
