Evaluation of the retinal differentiation ability of human induced pluripotent stem cells reprogrammed from different somatic cells
10.3760/cma.j.cn115989-20200805-00563
- VernacularTitle:不同体细胞来源人诱导多能干细胞向视网膜类器官分化能力的评价
- Author:
Bingbing XIE
1
;
Yuanyuan GUAN
;
Guanjie GAO
;
Dandan ZHENG
;
Ping XU
;
Xiaojing SONG
;
Xiufeng ZHONG
Author Information
1. 中山大学中山眼科中心 眼科学国家重点实验室，广州 510060
- From:
Chinese Journal of Experimental Ophthalmology
2020;38(10):821-827
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the retinal differentiation ability of human induced pluripotent stem cells (hiPSCs) from various somatic cell sources.Methods:The hiPSCs lines BC1- green fluorescent protein (GFP) and Gibco obtained by blood cell reprogramming and the hiPSCs line UE017 obtained by urine cell reprogramming were used to induce retinal differentiation.The morphogenesis and development of retina were recorded with an optical microscope, and the expression of specific molecular markers of various cell subclasses in the retina was detected by immunofluorescence, and the efficiency of retinal differentiation of different cell lines was analyzed and compared.Results:All three hiPSC lines derived from blood and urine cells were able to be induced into three-dimensional (3D) retinal organoids, including neuroretina and retinal pigment epithelial cells.Retinal organoids simulated the development process of retina in vivo and gradually differentiated into all cell subtypes of retina, including retinal ganglion cells, photoreceptor cells, amacrine cells, horizontal cells, bipolar cells, Müller cells, and even formed lamellar structures.However, in terms of the efficiency of acquiring retinal organoids, the hiPSCs derived from blood were more efficient than those derived from urine. Conclusions:hiPSCs from both blood and urine somatic cells can differentiate into 3D retinal organoids, including all subtypes of retinal cells.The differentiation efficiency among lines is different.
