In vitro interactions between antifungals and tacrolimus against Fusarium solani
10.3760/cma.j.cn115989-20190613-00260
- VernacularTitle:抗真菌药物单用与联合应用他克莫司对镰刀菌体外抑菌作用的比较
- Author:
Xiaolian CHEN
1
;
Hui DING
;
Hongshan LIU
;
Hong HE
;
Jiaochan WU
;
Xianlyu HU
;
Xingwu ZHONG
Author Information
1. 中山大学中山眼科中心海南眼科医院 海南省眼科医院 海南省眼科学重点实验室，海口 570311
- From:
Chinese Journal of Experimental Ophthalmology
2020;38(8):653-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the in vitro interaction between antifungals and tacrolimus against Fusarium solani. Methods:According to Clinical and Laboratory Standards Institute(CLSI) M27-Ed4 and M38-A3, 24 strains of Fusarium solani isolated from clinic were used to test the minimal inhibitory concentration (MIC) of the four antifungals.Chessboard microdilution method was used to evaluate the interaction of tacrolimus (FK506) combined with four antifungals in vitro.The study protocol was approved by the Ethics Committee of Hainan Eye Hospital (No.2017-006). Results:The MIC ranges of fluconazole, voriconazole, natamycin and amphotericin B against 24 strains of Fusarium were 64-256 μg/ml, 1-8 μg/ml, 2-8 μg/ml and 1-8 μg/ml, respectively.Tacrolimus combined with fluconazole, voriconazole, natamycin and amphotericin B had synergistic effects on 12.5% (3/24), 4.2% (1/24), 16.7% (4/24) and 29.2% (7/24) Fusarium, respectively.There were no antagonistic effect in this study. Conclusions:Fusarium is sensitive to natamycin in vitro and partly sensitive to amphotericin B and voriconazole.Synergistic effects exist in few strains of Fusarium but no antagonistic effect.
