Application of single-cell RNA sequencing in visual system
10.3760/cma.j.cn115989-20200509-00325
- VernacularTitle:单细胞转录组测序在视觉系统研究中的应用
- Author:
Yifan LIU
1
;
Yin SHEN
Author Information
1. 武汉大学人民医院眼科中心 430060
- From:
Chinese Journal of Experimental Ophthalmology
2020;38(8):704-709
- CountryChina
- Language:Chinese
-
Abstract:
Single-cell RNA sequencing (scRNA-seq) is an emerging technology that observes transcriptomes' differences between individual cells at the cellular level.The basic strategy is to capture single cells and lysis the cells to get mRNA, then amplify them after reversing transcriptions, finally cDNA is used to build sequencing libraries.At present, this technology has been widely used in many subject areas.Visual system includes retina, lateral geniculate nucleus, and visual cortex, etc.It's responsible for the acquisition and processing of visual information and the formation of vision.Visual information accounts for more than 70% of all sensory information.Therefore, the study of the visual system is particularly important.With the rapid development of science and technology, there are more and more research results on scRNA-seq.This technology is gradually becoming an important tool for guiding clinical practice, and has provided a bridge for basic research transforming to clinical research.In this review, we introduce the main technical routes and methods of scRNA-seq and detail its application in visual system.
