Gene Expression Analysis of Inflammatory Cytokines in Korean Psoriatic Patients.
- Author:
Ji Hoon CHUN
1
;
Young Hoon YOON
;
Yong Beom CHOE
;
Lae Hyung KANG
;
Soon Young PAIK
;
Chul Jong PARK
Author Information
- Publication Type:Original Article
- Keywords: Cytokines; Gene expression; Psoriasis
- MeSH: Cytokines*; Gene Expression*; Healthy Volunteers; Humans; Interleukin-12 Subunit p40; Interleukin-17; Phenotype; Polymerase Chain Reaction; Population Characteristics; Psoriasis; Reverse Transcription; RNA, Messenger
- From:Annals of Dermatology 2017;29(4):422-426
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Although phenotypic heterogeneity of psoriasis is suggested by the alternate activation of either T-helper (Th)1-related or Th17-related cytokines, little is known about the mRNA levels of inflammatory cytokines. OBJECTIVE: To investigate whether there is differential expression of Th1-related and Th17-related inflammatory cytokine genes 1) between psoriatic patients and healthy controls, and 2) between patients with different psoriasis phenotypes. METHODS: Twenty-five patients with psoriasis (10 with guttate psoriasis and 15 with plaque psoriasis) and 5 healthy volunteers were enrolled in this study. The mRNA levels of circulating cytokines (interleukin [IL]-2, IL-12p40, interferon-γ, IL-17A, IL-22, and IL-23R) were measured by real-time reverse transcription polymerase chain reaction. RESULTS: The comparison between psoriatic and healthy control samples revealed that IL-12p40, IL-17A, and IL-22 mRNA levels were significantly higher (approximately 4∼6 folds) in the patients with psoriasis. The mRNA levels of these six cytokines in the blood did not differ between the guttate and plaque psoriasis groups. CONCLUSION: We found that the mRNA levels of blood inflammatory cytokines (IL-12p40, IL-17A, and IL-22) were significantly elevated in patients with psoriasis compared to the levels in healthy controls, but they did not significantly differ between patients with guttate and plaque type psoriasis.
