Performance of the Real-Q EBV Quantification Kit for Epstein-Barr Virus DNA Quantification in Whole Blood.
10.3343/alm.2017.37.2.147
- Author:
Hee Jae HUH
1
;
Jong Eun PARK
;
Ji Youn KIM
;
Sun Ae YUN
;
Myoung Keun LEE
;
Nam Yong LEE
;
Jong Won KIM
;
Chang Seok KI
Author Information
1. Department of Laboratory Medicine and Genetics, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea. changski@skku.edu
- Publication Type:Brief Communication ; Evaluation Studies
- Keywords:
Epstein-Barr virus;
Real-time PCR;
Quantification;
Whole blood;
Performance
- MeSH:
DNA, Viral/*blood/metabolism;
Epstein-Barr Virus Infections/diagnosis/virology;
Herpesvirus 4, Human/*genetics/isolation & purification;
Humans;
Limit of Detection;
Reagent Kits, Diagnostic;
Real-Time Polymerase Chain Reaction
- From:Annals of Laboratory Medicine
2017;37(2):147-150
- CountryRepublic of Korea
- Language:English
-
Abstract:
There has been increasing interest in standardized and quantitative Epstein-Barr virus (EBV) DNA testing for the management of EBV disease. We evaluated the performance of the Real-Q EBV Quantification Kit (BioSewoom, Korea) in whole blood (WB). Nucleic acid extraction and real-time PCR were performed by using the MagNA Pure 96 (Roche Diagnostics, Germany) and 7500 Fast real-time PCR system (Applied Biosystems, USA), respectively. Assay sensitivity, linearity, and conversion factor were determined by using the World Health Organization international standard diluted in EBV-negative WB. We used 81 WB clinical specimens to compare performance of the Real-Q EBV Quantification Kit and artus EBV RG PCR Kit (Qiagen, Germany). The limit of detection (LOD) and limit of quantification (LOQ) for the Real-Q kit were 453 and 750 IU/mL, respectively. The conversion factor from EBV genomic copies to IU was 0.62. The linear range of the assay was from 750 to 10⁶ IU/mL. Viral load values measured with the Real-Q assay were on average 0.54 log₁₀ copies/mL higher than those measured with the artus assay. The Real-Q assay offered good analytical performance for EBV DNA quantification in WB.
