Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo.
10.4062/biomolther.2015.045
- Author:
Jin Sung PARK
1
;
Hyun Jae LEE
;
Dong Yeong LEE
;
Ho Seung JO
;
Jin Hoon JEONG
;
Dong Hee KIM
;
Dae Cheol NAM
;
Choong Jae LEE
;
Sun Chul HWANG
Author Information
1. Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea. hscspine@hanmail.net
- Publication Type:In Vitro ; Original Article
- Keywords:
Osteoarthritis;
Wogonin;
Chondrocyte;
Metalloproteinase
- MeSH:
Animals;
Blotting, Western;
Caseins;
Chondrocytes;
Collagen Type II;
Gene Expression;
Interleukin-1beta;
Knee;
Models, Theoretical*;
Osteoarthritis*;
Rats;
Thrombospondins
- From:Biomolecules & Therapeutics
2015;23(5):442-448
- CountryRepublic of Korea
- Language:English
-
Abstract:
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1beta (IL-1beta)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1beta-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.
