Dengue Virus Serotypes Circulating in Khyber Pakhtunkhwa Province, Pakistan, 2013-2015.
10.3343/alm.2017.37.2.151
- Author:
Muhammad SULEMAN
1
;
Rani FARYAL
;
Muhammad Masroor ALAM
;
Salmaan SHARIF
;
Shahzad SHAUKAT
;
Uzma Bashir AAMIR
;
Adnan KHURSHID
;
Mehar ANGEZ
;
Massab UMAIR
;
Mian Muhammad SUFIAN
;
Yasir ARSHAD
;
Syed Sohail Zahoor ZAIDI
Author Information
1. Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
- Publication Type:Brief Communication
- Keywords:
Dengue;
Pakistan;
Epidemiology;
Real-time RT-PCR;
Serotypes
- MeSH:
Adolescent;
Adult;
Dengue/diagnosis/*epidemiology/virology;
Dengue Virus/genetics/*isolation & purification;
Disease Outbreaks;
Female;
Humans;
Male;
Middle Aged;
Pakistan/epidemiology;
RNA, Viral/genetics/metabolism;
Real-Time Polymerase Chain Reaction;
Serogroup;
Young Adult
- From:Annals of Laboratory Medicine
2017;37(2):151-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa (KPK) province. In this study, we determined the circulating dengue virus (DENV) serotypes using real-time reverse transcriptase (RT)-PCR to understand the serotype-based epidemiology of DENV. All four serotypes (DENV-1 [6%], DENV-2 [33%], DENV-3 [47%], and DENV-4 [0.1%]) were found circulating during the study period. Our findings suggest the need for an active surveillance system coupled with the laboratory diagnosis, especially in the chronic endemic areas of the country. Public awareness programs are needed for effective control and prevention of outbreaks in the future.