Analysis of expression of NADH dehydrogenase 1, NADH dehydrogenase 3 and taste 2 receptor member 43 genes in severe pneumonia children
10.3760/cma.j.cn101070-20190530-00468
- VernacularTitle:重症肺炎患儿NADH脱氢酶1、NADH脱氢酶3和味觉受体2家族成员43基因表达的意义
- Author:
Yang LIU
1
;
Li YANG
;
Lingxiao WANG
;
Lizhen GAO
Author Information
1. 驻马店市中心医院儿科，河南 驻马店 463000
- From:
Chinese Journal of Applied Clinical Pediatrics
2020;35(13):1019-1021
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the association of expression of NADH dehydrogenase 1 ( ND1), NADH dehydrogenase 3 ( ND3) and taste 2 receptor member 43 ( TAS2R43) genes with severe pneumonia in children. Methods:Thirty severe pneumonia children in the intensive care unit of Zhumadian Central Hospital from May 2016 to May 2018 were enrolled as the severe pneumonia group and 25 healthy children undergoing health examinations in Zhumadian Central Hospital over the same period were selected as the control group.The severe pneumonia group contained 17 boys and 13 girls, with an age of 5.30±1.69.The control group included 13 boys and 12 girls, with an age of 4.96±1.31.Expression of ND1, ND3 and TAS2R43 genes was detected by real-time PCR, and the relative expression of ND1, ND3 and TAS2R43 genes was calculated by 2 -ΔΔCt method. Results:The circulating threshold (Ct) values of ND1, ND3 and TAS2R43 mRNA in the severe pneumonia group were 20.49±0.45, 21.32±0.61 and 32.20±0.46, respectively, and those in the healthy control group were 26.69±0.62, 27.50±0.35 and 26.69±0.49, respectively.The difference between the 2 groups was statistically significant ( t=-14.02, -15.25, -14.19, all P<0.05). The relative expre-ssion of ND1, ND3 and TAS2R43 genes calculated by the 2 -ΔΔCt method in the severe pneumonia group was 51.27 folds, 50.56 folds and 0.02 folds as many as that in the control group. Conclusions:The expression of ND1, ND3 and TAS2R43 genes in severe pneumonia children is abnormal.These 3 genes may be closely related to severe pneumonia in children.
