Micro-computed tomography evaluation and pathological analyses of female rats with collagen-induced arthritis.
10.4142/jvs.2015.16.2.165
- Author:
Young Hee KIM
1
;
Jin Seok KANG
Author Information
1. Department of Biomedical Laboratory Science, Namseoul University, Cheonan 330-707, Korea. kang@nsu.ac.kr
- Publication Type:Comparative Study ; Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
arthritis;
collagen;
micro-computed tomography;
pathology
- MeSH:
Animals;
Arthritis, Experimental/chemically induced/*diagnosis;
Disease Models, Animal;
Female;
Pathology, Clinical;
Random Allocation;
Rats;
Rats, Wistar;
*X-Ray Microtomography
- From:Journal of Veterinary Science
2015;16(2):165-171
- CountryRepublic of Korea
- Language:English
-
Abstract:
Imaging techniques have been introduced to assess the efficacy and toxicity of developing pharmaceuticals. The purpose of this study was to perform a comprehensive characterization of collagen-induced arthritis (CIA) in rats using micro-computed tomography (micro-CT) and to compare the results with data from conventional pathological examination. Arthritis was induced by collagen in 24 female Wistar rats. Micro-CT and pathological analyses were performed to assess arthritis progression. Micro-CT analysis showed marked joint destruction occurring in a time-dependent manner following collagen administration. Bone volume was significantly decreased in the tibia at weeks 3 and 4 compared to week 0 (p < 0.05 and p < 0.01, respectively). Additionally, percent bone volume was significantly reduced in the tibia at week 4 compared to week 0 (p < 0.05). In contrast, bone surface/bone volume and trabecular separation were significantly increased in the tibia of the animals at week 4 compared to week 0 (p < 0.05). Severe joint destruction with extensive inflammation, erosion of cartilage and bone, and infiltration of inflammatory cells were observed in the knee joints of the collagen-treated rats. Taken together, micro-CT made it possible to quantify CIA lesions and should be performed with pathological examination in rats.
