In vitro development of canine somatic cell nuclear transfer embryos in different culture media.
10.4142/jvs.2015.16.2.233
- Author:
Dong Hoon KIM
1
;
Jin Gu NO
;
Mi Kyung CHOI
;
Dong Hyeon YEOM
;
Dong Kyo KIM
;
Byoung Chul YANG
;
Jae Gyu YOO
;
Min Kyu KIM
;
Hong Tea KIM
Author Information
1. Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea. kimdhhj@korea.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
canine;
G1/G2 media;
in vitro culture;
somatic cell nuclear transfer embryo
- MeSH:
Animals;
Blastocyst/cytology;
Cloning, Organism/*veterinary;
Culture Media/metabolism;
Dogs/*embryology;
Embryo Culture Techniques/*veterinary;
*Embryonic Development;
Nuclear Transfer Techniques/*veterinary
- From:Journal of Veterinary Science
2015;16(2):233-235
- CountryRepublic of Korea
- Language:English
-
Abstract:
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
