miR-483-5p promote adrenocortical cancer proliferation and invasion via down-regulation of CDK15
10.3760/cma.j.issn.115807-20200110-00007
- VernacularTitle:miR-483-5p通过下调CDK15促进肾上腺皮质癌增殖和侵袭
- Author:
Guannan WANG
1
;
Zhiwei XU
;
Tingting HE
;
Ping ZHOU
;
Jianping MA
;
Huimin LONG
Author Information
1. 宁波市医疗中心李惠利医院血透室 315000
- From:Chinese Journal of Endocrine Surgery
2020;14(2):155-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of miR-483-5p in adrenocortical cancer (ACC) and its possible mechanism.Methods:Quantitative real-time polymerase chain reaction was conducted to estimate the expression of miR-483-5p and CDK15 in ACC tissues and cell lines. The effects of miR-483-5p on proliferation were determined in vitro using CCK-8 proliferation assays, the changes of invasion of ACC cells were examined by Transwell. The molecular mechanism underlying the relevance between miR-483-5p and CDK15 was confirmed by luciferase assay and rescue assays.Results:We found a relatively higher miR-483-5p (2.36±1.02 vs 1.09±0.43) and lower CDK15 (0.57±0.26 vs 1.06±0.32) expression in ACC specimens and cell lines. CDK15 was verified as a direct target of miR-483-5p by luciferase assay. over-expression of miR-483-5p promoted proliferation (24 h: 0.26±0.03 vs 0.23±0.04, 48 h: 0.56±0.05 vs 0.41±0.03, 72 h: 0.73±0.04 vs 0.59±0.03) and invasion (95.78±4.66 vs 23.89±2.52) by down-regulating CDK15 expression.Conclusion:miR-483-5p plays a tumorigenesis role in ACC progression by down-regulating CDK15 expression, which may lead to a novel insight to the potential biomarker and novel therapeutic strategies for ACC.
