Effect of microRNA125a on macrophage polarization during myocardial ischemia reperfusion
10.3760/cma.j.issn.1671-0282.2020.02.0025
- VernacularTitle:microRNA125a对缺血-再灌注心肌组织中巨噬细胞极化的影响
- Author:
Dongna GUO
1
;
Bing JI
;
Lijuan GENG
;
Qiongjie LI
;
Yunjun WANG
Author Information
1. 山西医科大学附属第一医院急诊医学中心，030001 太原
- From:
Chinese Journal of Emergency Medicine
2020;29(2):243-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of microRNA125a in infiltrating macrophages during myocardial ischemia reperfusion, and to analyze the effect of microRNA125a on the polarization of infiltrating macrophages during myocardial ischemia reperfusion.Methods:The rat model of myocardial ischemia-reperfusion was established. Myocardial injury tissues were taken, infiltrated macrophages were isolated, and RNA was extracted. qRT-PCR was used to detect M1 and M2 markers in macrophages and evaluate the types of infiltrated macrophages. Subsequently the expression level of microRNA125a in infiltrating macrophages was detected. Finally, the effect of microRNA125a on macrophage polarization was further elucidated.Results:Macrophages in myocardium of ischemia reperfusion showed high expression of M1 markers (TNF-alpha and IL-8), presenting M1 macrophages. At the same time, microRNA125a was also highly expressed in this macrophage. Knockdown or overexpression of microRNA125a in macrophages in vitro can affect the macrophages polarization.Conclusions:Macrophages in myocardial ischemia reperfusion are M1 type, and microRNA125a is highly expressed in this macrophage and promotes its migration to M1 type.
