Effect of thymosin α1 on immune cell differentiation in sepsis mice
10.3760/cma.j.issn.1671-0282.2020.02.0015
- VernacularTitle:胸腺肽α1对脓毒症小鼠免疫细胞分化的影响
- Author:
Zhiqiang ZHU
1
;
Changju ZHU
;
Hui PEI
;
Jiafeng XIE
;
Yepeng ZHANG
Author Information
1. 郑州大学第一附属医院急诊医学部，郑州 450000
- From:
Chinese Journal of Emergency Medicine
2020;29(2):204-209
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of thymosin α1 on the differentiation of T lymphocyte and the secretion of inflammatory factors in septic mice, thus to explore the effect of thymosin α1 on the prognosis of sepsis.Methods:Adult female C57 mice were randomly (random number) divided into 3 groups: blank control group, sepsis group, and thymosin α1 treatment group. T cell counts and the corresponding inflammatory factors in the further differentiation of T lymphocytes as well as plasma and lung tissues were statistically analyzed, and the survival rate of the mice within 96 h was also analyzed. Graphpad 7.0 software was used for statistically analysis of the study results.Results:There was no significant difference in T cell counts among the three groups of mice, but in the further differentiation of T lymphocytes, the expression of Th17 in the thymosin α1 treatment group was significantly lower than that in the sepsis group, and the expression of Treg was significantly increased in the sepsis group. The expression of the inflammatory cytokine IL-10 was significantly increased in plasma and lung tissues of the thymosin α1 treatment group, while the expression of IL-17A in plasma and lung tissues of the thymosin α1 treatment group was significantly lower ( P <0.05). Survival analysis showed that the survival rate of the thymosin α1 treatment group increased significantly at 96 h, and the difference was significant statistically ( P <0.05). Conclusions:Thymosin α1 can enhance the cellular immunity in sepsis, ameliorate the systemic inflammation, and further protect against sepsis.
