Content determination of Chikusetsu saponin Ⅳa of root, stem and leaf in Panacis japonici rhizoma
10.3760/cma.j.cn115398-20191014-00081
- VernacularTitle:竹节参不同部位竹节参皂苷Ⅳa含量测定
- Author:
Xingyan SU
1
;
Yanmei WU
;
Dandan XU
;
Xing TU
Author Information
1. 湖北省恩施土家族苗族自治州中心医院麻醉科 445000；长江大学医学部，荆州 434000
- From:
International Journal of Traditional Chinese Medicine
2020;42(9):900-903
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the content of Chikusetsu saponin Ⅳa in the roots, stems and leaves in Panacis japonici rhizoma, and explore its development value. Methods:By using High Performance Lliquid Chromatography (HPLC) method to determine the content of Chikusetsu saponin Ⅳa in the roots, stems and leave of Panacis japonici rhizoma. Inertsil ODS-sp (4.6 mm×150 mm, 5 μm) was used as the column and the acetonitrile-0.2% phosphoric acid (35:65) as the mobile phase. The flow rate was 1 ml/min with the temperature 35 ℃ under wavelength 210 nm. Results:The linear ranged from 1-10 μg of Chikusetsu saponin Ⅳa was good ( r=0.999 5). The content of Chikusetsu saponin Ⅳa in the roof, stem and leaf were 2.169%, 0.269%, 0.169%, respectively. Conclusions:The roof of Panacis japonici rhizoma contains the highest level of Chikusetsu saponin Ⅳa followed by stem and leaf. The roof part could be used as the medical part and its stem and leaf are also valuable for further development.
