Identification research with differential scanning calorimetry method between gelatin Chinese herbal slices and formula granules
10.3760/cma.j.cn115398-20191216-00127
- VernacularTitle:差示扫描量热法用于胶类中药饮片及配方颗粒的鉴别研究
- Author:
Jinzhi SHEN
1
;
Huamei SU
;
Yuchun SU
Author Information
1. 厦门大学附属中山医院药学部 361004
- From:
International Journal of Traditional Chinese Medicine
2020;42(7):683-687
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a simple and fast differential scanning calorimetry (DSC) method for identifying different gelatin Chinese herbal slices and formula granules.Methods:Adopting DSC method with 100 mesh sample size, making aluminum crucible as the reference, nitrogen was atmosphere, and the flow rate was 100 ml/min. The optimal temperature increasing range and temperature increasing rate are investigated. Based on the optimized DSC test conditions, 42 batches of Asini corii colla, Cervi cornus colla, Testudinis carapacis and formula particles were identified.Results:The optimal heating range of DSC test was 30-500 ℃, and the heating rate was 30 ℃/min. There were 4, 3, 2 aracteristic peaks of Asini corii colla, Cervi cornus colla, and Testudinis carapacis et plastri colla, and their peaks' shapes and positions were different. The antler gum formula particles and tortoise shell formula particles had two exothermic peaks, but the peak positions were different. There was one exothermic peak in the Asini corii colla. There is an exothermic peak in the traditional Chinese medicine decoction pieces and formula particles of antler gum formula particles at about 325 ℃. This exothermic peak can be used as the characteristic peak of antler gum formula particles.Conclusions:DSC has the advantages of simple operation, small amount, no reagents, fast measurement, good reproducibility, and easy analysis of the spectrum. And it can identify the value and size to distinguish between various gel pieces and formula granules.
