Identification of the chemical constituents of Fangfeng-Tongsheng Pills by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry
10.3760/cma.j.cn115398-20190817-00121
- VernacularTitle:基于超高效液相色谱-四级杆/静电场轨道阱高分辨质谱技术的防风通圣丸化学成分研究
- Author:
Wenning YANG
1
;
Qianwan MENG
;
Wenshuai TIAN
;
Dandan ZHOU
Author Information
1. 鉴甄检测技术（上海）有限公司 200131；北京中医药大学中药学院 102488
- From:
International Journal of Traditional Chinese Medicine
2020;42(7):670-678
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the chemical constituents of Fangfeng-Tongsheng Pills by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Orbitrap HRMS). Methods:Take Agilent InfinityLab Poroshell 120 C18 column (150 mm×3.0 mm, 2.7 μm) and make water containing 0.1% formic acid and acetonitrile as gradient eluents. Data was collected with positive and negative ion modes using full scan and two-stage threshold-triggered mass modes (Full MS/dd-MS 2) and HESI source. The main chemical constituents of Fangfeng-Tongsheng Pills were identified according to the exact molecular mass, the cleavage fragments of MS 2, the retention time of reference substances, mzVault 2.0 MS database and literature review. Results:A total of 82 chemical constituents including 33 flavonoids, 13 chromones, 8 triterpenoid saponins, 6 coumarin, 5 iridoids, 4 phthalides, 4 terpenoids, 3 phenolic acids, 3 lignans, 2 anthraquinone and 1 alkaloids, were detected and identified.Conclusions:UPLC-Q-Orbitrap HRMS could identify the main chemical constituents of Fangfeng-Tongsheng Pills rapidly and accurately, which may provide a theoretical basis for further studies on the mechnism and quality control of this formula.
