Research on the determination of emodin in Xiaoyan granulas
10.3760/cma.j.cn115398-20190612-00069
- VernacularTitle:消炎颗粒中大黄素的含量测定研究
- Author:
Fuzhou CHEN
1
;
Ni LI
;
Bilin DENG
;
Kai XIAO
Author Information
1. 南昌市结石病专科医院药剂科 330001
- From:
International Journal of Traditional Chinese Medicine
2020;42(4):372-374
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of emodin in Xiaoyan granulas by high performance liquid chromatography (HPLC). Methods:Took inertsil ODS-3 (250 mm × 4.6 mm, 5 μm) as the chromatographic separation the mobile phase was acetonitrile-0.1% phosphoric acid (80:20), the detection wavelength was 254 nm, the flow rate was 1.0 ml/min, and the column temperature was at 40 ℃.Results:The linear range of emodin was 0.034 6-0.432 0 μg ( r=0.999 9). The average recovery was 94.02% ( RSD=1.29%, n=6). Conclusions:This method is simple, accurate, which could be used repeatedly and can be used as the emodin determination method for Xiaoyan granulas.
