Quality evaluation research of compound Danshen tablets by real-time cell-based assay technology
10.3760/cma.j.cn115398-20190624-00155
- VernacularTitle:基于实时细胞电子分析技术的复方丹参片质量评价研究
- Author:
Xiaofei MA
1
;
Guojun YAN
;
Jinhuo PAN
;
Jun CHEN
;
Qian ZHANG
;
Xingxing HAN
;
Jianchun WANG
Author Information
1. 南京中医药大学药学院 210023
- From:
International Journal of Traditional Chinese Medicine
2020;42(3):251-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Real-time cell-based assay (RTCA) technology was used to detect the influence of compound Danshen tablets with different concentrations on cells to screen outthe sensitive, stable and concentration-dependent cell lines to provide reference for the quality evaluation experiment of cell biological effects of compound salvia miltiorrhiza tablets. Methods:The effects of compound Danshen tablets on human umbilical vein endothelial cells (Huvec), rat cardiac myocytes (H9C2) and rat thoracic aortic smooth muscle cells (RA-VSMC) were investigated by RTCA. The cell index (CI) was measured to form time-dose dependent cell response curves (TCRPs) to screen the the optimal cell lines by description analysis and statistical analysis. Results:All of the three cells show obvious response to the effects of compound Danshen tablets. The sensitivity of RA-VSMC was relatively poor, the range of effective concentrationof three cells was different, and the concentration gradient dependence was also different. H9c2 cells had good sensitivity to compound Danshen tablets, which was gradient dependent, and the effective concentration range wass 0.3-1.8 mg/ml, and it can be used as a cell for quality evaluation of compound Danshen tablets. Conclusions:RTCA technology can monitor and record the growth status of cells in real time. It can accurately reflect the effects of compound Danshen tablets on different cell lines. The data are objective and reliable. It provides a new experimental technology and method for the quality evaluation of compound Danshen tablets.
