Simultaneous determination of seven constituents in Ermu-Ningsou Pills by UPLC with wavelength switching method
10.3760/cma.j.cn115398-20190727-00134
- VernacularTitle:超高效液相色谱波长切换法同时测定二母宁嗽丸中7个成分含量
- Author:
Gongjing LU
1
;
Anfeng HUANG
Author Information
1. 山东省东营市食品药品检验中心生物检验科 257091
- From:
International Journal of Traditional Chinese Medicine
2020;42(3):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method for simultaneous determination of the contents of geniposide, hesperidin, baicalin, liquiritin, glycyrrhetinic acid, glycyrrhizic and schizandrin in Ermu-Ningsou Pills by ultra performance liquid chromatography (UPLC) with wavelength switching. Methods:The analyses were carried out on an Phenomenex Kinetex C18 column (4.6 mm×100 mm, 2.7 μm) with the mobile phase consisting of methanol(A)-0.05% phosphoric acid (B) in a gradient mode at a flow rate of 0.6 ml/min. The detection wavelength was set at 237 nm for geniposide, liquiritin, glycyrrhetinic acid and glycyrrhizic, 280 nm for baicalin and hesperidin, 250 nm for schizandrin. The column temperature was set at 35 ℃ and the injection volume was 2 μl.Results:The linear relation of geniposide, hesperidin, baicalin, liquiritin, glycyrrhetinic acid, glycyrrhizic and schizandrin were excellent within the range of 10.294-205.888, 4.552-91.036, 6.212-124.248, 8.974-179.484, 2.629-52.580, 5.371-107.416, 8.905-178.104 ng ( r≥0.999 5, n=6). Average recoveries were 98.47%, 99.04%, 100.76%, 98.27%, 100.50%, 98.79%, 99.37% ( RSD<2.0%, n=6). RSDs of precision, reproducibility and stability tests (24 h) were lower than 2.0% ( n=6). Conclusions:The method is simple, effective, accurate. It can be used for simultaneous determination of 7 active constituents in Ermu-Ningsou Pills.
