Study on the inhibitory effect of CPT combined with chemotherapy drugs on gastric carcinoma cells in vitro
10.3760/cma.j.cn115396-20200426-00117
- VernacularTitle:CPT联合化疗药物对胃癌细胞体外抑制作用研究
- Author:
Zhicheng GE
1
;
Xuemei MA
;
Jun ZHANG
;
Zhongtao ZHANG
;
Yu WANG
Author Information
1. 首都医科大学附属北京友谊医院普外科 100050
- From:
International Journal of Surgery
2020;47(5):329-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effect of CPT combined with 5-Fluorouracil (5-Fu), Oxaliplatin(L-OHP) on gastric carcinoma cells in vitro. Methods:MGC823 and SGC7901 gastric carcinoma cell lines were selected for culture and passage. The two cell lines were distributed into blank control group, CPT group, 5-Fu group, L-OHP group, 5-Fu+ CPT group and L-OHP+ CPT group. The blank control group was not given any drugs, CPT group was given CPT 1 000 μg/ml 2-fold dilution until 0.004 μg/ml; 5-Fu group was given 5-Fu 2.5×10 6 ng/ml 10-fold dilution until 2.5×10 -4 ng/ml; L-OHP group was given L-OHP 1 000 μg/ml 2-fold dilution up to 0.002 μg/ml; 5-Fu+ CPT group was given 5-Fu step concentration 2.5×10 6 ng/ml 10-fold dilution up to 2.5×10 -8 ng/ml, and combined with CPT 300 ng/ml; L-OHP+ CPT group was given a 2-fold dilution of L-OHP step concentration 1 000 μg/ml up to 0.24 μg/ml and combined with CPT 300 ng/ml. The inhibition effect of cells after drug action was detected. The 50% inhibitive concentration (IC 50) of medicines and the synergistic effect of the combined drugs were analyzed. The measurement data were expressed as Mean ± standard deviation ( Mean± SD), and the t-test was used for comparison between groups. Results:The inhibition rate of 5-Fu+ CPT group was significantly higher than that of 5-Fu group, the differences in MGC823 and SGC7901 cell lines were statistically significant ( P=0.002, 0.009). The inhibition rate of L-OHP+ CPT group was also significantly higher than that of L-OHP group, the differences in MGC823 and SGC7901 cell lines were statistically significant ( P=0.037, 0.040). Compared with 5-Fu+ L-OHP group, IC 50 value of 5-Fu+ CPT group and L-OHP+ CPT group decreased significantly, the differences in MGC823 and SGC7901 cell lines were statistically significant (all P<0.001). The survival fraction of 5-Fu and L-OHP combined with CPT on the two cell lines was lower than 70%, which was estimated by Weeb coefficient. Conclusions:The combination of 5-Fu, L-OHP and CPT can significantly improved the inhibitory effect on MGC823 and SGC7901 cell lines. 5-Fu, L-OHP and CPT had good synergistic effect.
