Functional mechanism of circ_0009910 in gastric cancer
10.3760/cma.j.cn115396-20191125-00223
- VernacularTitle:circ_0009910在胃癌细胞中作用机制初步研究
- Author:
Ming LIU
1
;
Le ZHANG
;
Kaidong LIU
;
Yuekui BAI
Author Information
1. 北京市海淀医院普通外科 100080
- From:
International Journal of Surgery
2020;47(4):240-245,289
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explored the mechanism of circ_0009910 in gastric cancer cells by detecting and analyzing the expression of it.Methods:qRT-PCR assay was used to detect the expression level of circ_0009910 in gastric cancer cell lines BGC823, SGC7901, AGS, MGC803 and MKN45. In circumstance, circ_0009910 siRNA was used to knock down circ_0009910 in BGC823 and AGS cells. The transfection efficiency was verified. In BGC823 and AGS cells knocked down by circ_0009910, cell proliferation was detected by MTT assay, colony formation was detected by plate assay, migration and invasion were detected by Transwell, and epithelial-mesenchymal transition (EMT) was detected by western-blotting. The software of SPPSS 18.0 was used to conduct statistical analysis.Results:The relative expression levels of circ_0009910 in BGC823, SGC7901, AGS, MGC803, and MKN45 were (7.238±0.895), (5.023±0.786), (4.184±0.356), (8.561±1.026), (3.478±0.301), which were significantly higher than normal gastric cancer ( P<0.01). After knocking down circ_0009910 by siRNA transfection, the BGC823, AGS cell viability, colony formation number, migration and invasion ability were significantly reduced compared with the control group ( P<0.01), the relative expressions of N-cadherin and Snail proteins were reduced, and E -Cadherin expression increased. Conclusion:Circ_0009910 is highly expressed in gastric cancer cells, and can promote gastric cancer cell proliferation, colony formation, migration, invasion and EMT.
