The Pathogenesis of Adenomyosis Through the Immunohistochemical Study with bcl-2 and MMP-9.
- Author:
Myoung Seok HAN
1
;
Geun A SONG
;
Hyo Jeong JUN
;
Dae Cheol KIM
;
Jin Sook JEONG
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Dong-A University, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Adenomyosis;
bcl-2;
MMP-9
- MeSH:
Adenomyosis*;
Antibodies;
Cell Proliferation;
Endometrium;
Female;
Matrix Metalloproteinase 9
- From:Korean Journal of Obstetrics and Gynecology
2004;47(9):1714-1718
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To understand the pathogenesis of adenomyosis through the immunohistochemical staining with bcl-2 and matrix metalloproteinase-9 (MMP-9) antibody. METHODS: We selected 45 cases of adenomyosis among the uterine specimens that were extirpated surgically due to benign gynecologic problem. Immunohistochemical staining to the myometrial tissue was performed using anti-bcl-2 and anti-MMP-9 antibodies. Staining was qualitatively assessed in terms of extent and intensity. RESULTS: There were 32 cases of proliferative phase and 13 cases of secretory phase depending on the date of endometrium. Anti-bcl-2 stained 81.3% (26/32) compared with anti-MMP-9 stained 6.3% (2/32) in the glandular tissue of the proliferative phase (p<.001). In the secretory phase, anti-bcl-2 92.3% (12/13) and anti- MMP-9 7.7% (1/13) respectively (p<.001). In the stromal tissue of the proliferative phase, anti-bcl-2 stained 62.5% (20/32) compared with anti- MMP-9 stained 6.3% (2/32) (p<.001). In the secetory phase, anti-bcl-2 84.6% (11/13) and anti-MMP-9 15.4% (2/13) (p<.001). CONCLUSION: The adenomyosis may be developed from simple invagination and cellular proliferation of endometrial tissue, not caused by myometrial infiltration of endometrial tissue.
