Effects of intraperitoneal injection of insulin on the regulation of abnormal blood glucose and blood lipid in obese type 2 diabetic KKAy mice
10.3760/cma.j.cn121382-20200411-00504
- VernacularTitle:胰岛素腹腔注射对2型糖尿病KKAy小鼠异常血脂调节的影响
- Author:
Ting XIA
1
;
Guanxin GAO
;
Huilin XIA
;
Hongfan SUN
;
Lan YU
;
Lina DU
;
Jiahui SUN
;
Shiyu YANG
;
Jie CHANG
Author Information
1. 内蒙古自治区人民医院医学工程处，呼和浩特 010017
- From:
International Journal of Biomedical Engineering
2020;43(5):360-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of insulin intraperitoneal injection on abnormal blood lipid intype 2 diabetic KKAy mice.Methods:Type 2 diabetic mice model was established by feeding high fat and high sugar diet. KKAy model mice were randomly divided into intraperitoneal injection group ( n=6), subcutaneous injection group ( n=6) and no-treatment group ( n=3). At the same time, healthy C57BL/6J mice were selected as normal group ( n=6), and healthy KKAy mice as disease-free group ( n=6). The treatment process was divided into two stages. The first stage consists of 6 weeks, in which the mice in the intraperitoneal and subcutaneous groups were treated with insulin intraperitoneally and subcutaneously respectively. The second stage consists of 4 weeks, in which the mice in intraperitoneal and subcutaneous groups were subcutaneously injected with insulin. The mice in the remaining 3 groups were not treated. The changes of related indicators were detected every two weeks, including body weight, fasting blood sugar, 2 hours after meal blood sugar, triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C). Results:Changing the injection solution in the medium term of the treatment had no effect on the body mass and blood sugar of KKAy mice with type 2 diabetes. Under this condition, the effect of intraperitoneal injection of insulin on HDL-C and LDL-C is significantly better than that of subcutaneous injection. Besides, both injection solutions are effective in regulating TG, but the effect of reducing total cholesterol is not obvious.Conclusions:The intraperitoneal injection of insulin has a certain effect on the blood lipid abnormality of type 2 diabetic KKAy mice. It can promote the increase of HDL-C, the decrease of LDL-C, and the decrease of TG.
