Stability of immunomodulatory activity of aqueous extracts from wild and cultivated Artemisia rupestris L. in Xinjiang
10.3760/cma.j.cn121382-20200428-00501
- VernacularTitle:新疆野生和栽培一枝蒿粗多糖免疫调节活性的稳定性分析
- Author:
Quanxiao LI
1
;
Shuangshuang FENG
;
Ailian ZHANG
Author Information
1. 新疆大学生命科学与技术学院，新疆生物资源与基因工程重点实验室，乌鲁木齐 830046
- From:
International Journal of Biomedical Engineering
2020;43(5):341-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigated the effects of storage temperature and storage state on the immunoregulatory activities of wild Artemisia rupestris L. crude polysaccharides (WARCP) and cultivated Artemisia rupestris L. crude polysaccharides (CARCP). Explore the optimal storage conditions for WARCP and CARCP.Methods:WARCP and CARCP were stored at different temperatures (4, -20 and -80 ℃) and in different states (powder and solution) for 6 months. Different doses (10, 50, 100 μg/ml) of WARCP and CARCP were used to stimulate mouse bone marrow dendritic cells (DCs) for 24 h in vitro. The lipopolysaccharide with a dose of 100 ng/ml were used as positive control, and RPMI-1640 medium was used as negative control. Flow cytometry was used to detect the proportions of CD40 and CD86 on the surface of DCs.Results:At different temperatures, the differences in immunomodulatory activity of WARCP and CARCP stored in powder state and WARCP stored in solution state were not statistically significant (all P>0.05). The immunomodulatory activities of CARCP stored in solution state at -20 ℃ and -80 ℃ were significantly better than that stored at 4 ℃ (all P<0.05). Conclusions:After 6 months of storage, the immunomodulatory activity of WARCP and CARCP is not affected by the storage state, and can maintain good immunomodulatory activity at different storage temperatures.
