Effect of berberine on alveolar bone absorption in diabetic rats
10.3760/cma.j.cn121382-20200112-00301
- VernacularTitle:黄连素对糖尿病大鼠牙槽骨吸收的影响
- Author:
Ping WANG
1
;
Minhong HOU
Author Information
1. 天津市妇女儿童保健中心口腔科 300070
- From:
International Journal of Biomedical Engineering
2020;43(3):171-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect and mechanism of berberine intervention on alveolar bone absorption in diabetic rats.Methods:Diabetes rat model was established by one-time intraperitoneal injection of streptozotocin (35 mg/kg). Sixty healthy female Sprague-Dawley rats without specific pathogens were divided into normal group, diabetic group and berberine group according to the random number table method, 20 rats for each group. The rat models in the berberine group and the diabetic group were given berberine (200 mg/kg) and an equal volume of sodium chloride injection by gavage, once a day, for 4 consecutive weeks. The rat models in the normal group were not treated. Fasting blood glucose (FBG), fasting insulin level (Fins) , hormone resistance index (HOMA-IR) and insulin sensitivity index (ISI), as well as the serum calcium, phosphorus content, alkaline phosphatase activity and osteocalcin content of the rats were measured. The pathological changes of periodontal tissues were detected by HE staining. The expression of BMP-2/Smad pathway (BMP-2, Smad1, Smad4) was detected by RT-PCR and Western blot.Results:Compared with the diabetes group, the FBG level of rats in the berberine group [(9.88±1.23) mmol/L vs. (21.66±2.41) mmol/L], Fins levels [(11.63±3.63) mIU/L vs. (21.64±5.58) mIU/L] , HOMA-IR (7.58±0.96 vs. 20.85±1.95) were reduced, and ISI (-5.10±0.16 vs. 6.15±0.18), serum calcium levels[(1.855±0.125) mmol/L vs. (1.317±0.104)) mmol/L], serum phosphorus levels [(3.087±0.278) mmol/L vs. (2.526±0.226) mmol/L], serum ALP levels [(130.681±18.963) U/L vs. (99.874±31.968) U/L] and osteocalcin levels [(112.368±9.752) ng/ml vs. (78.645±10.973) ng/ml] were all increased, and the differences were statistically significant (all P<0.05). The results of hematoxylin-eosin staining showed that there was no bone resorption in the alveolar bone of the rats in the normal group, bone resorption was found in the alveolar bone of the rats in the diabetes group, and the bone resorption of the alveolar bone in the berberine group was significantly reduced. RT-PCR and Western Blot results showed that compared with the diabetes group, the BMP-2, Smad1, Smad4 gene mRNA and protein in the alveolar bone tissue of the berberine group were significantly increased (all P<0.05). Conclusions:Berberine intervention can reduce FBG levels in diabetic rats and improve insulin resistance, which may reduce alveolar bone resorption in diabetic rats by up-regulating the BMP-2/Smad signaling pathway.
