Loss of Heterozygosity at 3p in Korean Non-Small Cell Lung Cancer.
10.4046/trd.1998.45.5.975
- Author:
Choon Taek LEE
1
;
Mi Hee KIM
;
Kyung Ho PARK
;
Jong Ho PARK
;
Hee Jong BAEK
;
Jae Ill ZO
;
Jin Kyoo KIM
;
Chang Min KIM
Author Information
1. Department of Medicine, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
3p deletion;
Non-small cell lung cancer(NSCLC);
Microsatellite;
PCR-LOH
- MeSH:
Adenocarcinoma;
Carcinoma, Non-Small-Cell Lung*;
DNA;
Genes, Tumor Suppressor;
Humans;
Loss of Heterozygosity*;
Lung;
Lung Neoplasms;
Microsatellite Repeats;
Polymorphism, Restriction Fragment Length;
Smoke;
Smoking
- From:Tuberculosis and Respiratory Diseases
1998;45(5):975-983
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Frequent deletion of 3p in numerous cancer including lung cancer suggests the presence of tumor suppressor gene. 3p has been analysed by RFLP and PCR-LOH of microsatellite locus. In this study, we observed the deletion of 3p in Korean NSCLC by PCR-LOH of 4 microsatellite loci and investigated the clinical significance. Method : 62 surgically rejected NSCLC DNA and normal lung DNA have been analysed by PCR-LOH at three dinucleotide[D3S1228 (3p14.1-14.3), D3S1067 (3p14.3-21.1), D3S1029 (3p21.1-21.3)] and one tetranucleotide[D3S1537 (Sp 22-24.2)] repeat microsatellite loci. RESULTS: Among 59 informative cases, 3p deletion by PCR-LOH at four microsatellite loci was found in 31 patients (52.5%). 3p deletion were found in 55% of squamous cell lung cancer and 47% of adenocarcinoma patients. No significant difference has been found in clinical parameters such as staging, smoking and survival according to the status of 3p deletion. CONCLUSION: Deletions in 3p have played an important role in Korean NSCLC though no clinical significance was detected.
