Screening infant spasmodic differentially expressed protein based on iTRAQ technique
10.3760/cma.j.issn.1673-4408.2020.03.015
- VernacularTitle:基于iTRAQ技术筛选婴儿痉挛症差异表达蛋白的研究
- Author:
He ZHAO
1
;
Ziteng TENG
;
Hua WANG
Author Information
1. 中国医科大学附属盛京医院儿内科，沈阳 110004
- From:
International Journal of Pediatrics
2020;47(3):213-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen serum biomarkers of infantile spasm by iTRAQ technique.Methods:Fifteen cases of infants with infantile spasm and 15 cases of healthy infants were selected to find the plasma differential proteins by isobaric tag for relative and absolute quantitation(iTRAQ) technique and analysis of the biological information, then candidate proteins with significant differences were screened out.Results:Compared with the control group, 59 different proteins were obtained in the experimental group with a multiple of 1.2 times, which were involved in biological processes such as cytoskeleton organization, integrin activation and maintenance of protein location in cells.Five disease-related protein markers were selected and discussed.Conclusion:Differences in protein expression were found in plasma between infants with infantile spasm and normal controls.It may provide a new direction for the research of infant spasm.The research of cytoskeleton protein may become the breach of the infant spasm pathogenesis.New potential biomarkers, such as serine/threonine protein kinase and dopamine beta hydroxylase may provide a new direction for diagnosis and treatment of infant spasm.
