Effect of CUEDC1 gene on the gene expression profile of acute monocytic leukemia THP-1 cells based on RNA sequencing
10.3760/cma.j.cn115356-20200310-00062
- VernacularTitle:基于RNA测序研究CUEDC1基因对急性单核细胞白血病THP-1细胞基因表达谱的影响
- Author:
Xingxing CHAI
1
;
Xiaopeng DU
;
Fanjing MENG
;
Wanchuan ZHUANG
Author Information
1. 蚌埠医学院附属连云港医院血液内科，江苏 连云港 222023
- From:
Journal of Leukemia & Lymphoma
2020;29(11):648-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of CUEDC1 gene on the acute monocytic leukemia THP-1 cells gene expression profile.Methods:The differential expression gene bank of THP-1 cells with CUEDC1 gene interference was constructed. The differential gene expression of THP-1 cells in CUEDC1 interference group and the negative control group was compared based on RNA sequencing technology. The part of the differentially expressed genes were verified by using real-time polymerase chain reaction (PCR), and the up-regulated and down-regulated genes were respectively imported into DAVID software for gene ontology (GO) function enrichment analysis.Results:The differentially expressed gene bank of THP-1 cells interfered by CUEDC1 gene was successfully constructed. A total of 161 differentially expressed genes were detected in CUEDC1 interference group and the negative control group ( P < 0.05), including 85 up-regulated genes and 76 down-regulated genes. There were 9 genes related to cell proliferation and 10 genes related to apoptosis, 2 genes related to p53 gene and 3 genes related to transcriptional regulation, 8 genes related to ubiquitin, among which SMAD5, SG15, CBLL1, FANCF and other genes were closely related to the occurrence and development of acute leukemia. Conclusion:CUEDC1 gene participates in the occurrence and development of acute monocytic leukemia by influencing the expressions of SMAD5, SG15, CBLL1, FANCF and other genes.
