Comparison of the Legiolert enzyme-substrate method and the conventional cultivation for the detection of Legionella pneumophila in water samples from public places
- VernacularTitle:Legiolert酶底物法与传统培养法对公共场所水体样本中嗜肺军团菌检出结果的比较
- Author:
Yanli LI
1
;
Kang YANG
1
;
Lei FU
1
;
Lili GONG
1
;
Lanfang LIU
1
;
Jingzhi GAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Legiolert enzyme-substrate method; Legionella pneumophila; Conventional cultivation; Public places
- From: Journal of Public Health and Preventive Medicine 2021;32(1):51-54
- CountryChina
- Language:Chinese
- Abstract: Objective To compare the detection effects of Legionella pneumophila in water samples from public places by the Legiolert enzyme-substrate method and the conventional cultivation. Methods The Legiolert enzyme-substrate method and the conventional cultivation method were used to detect Legionella pneumophila in cooling water and shower water samples collected in public places. Isolated strains were verified and serotyped. Results A total of 68 samples were collected and tested. The positive rate of the conventional cultivation and the Legiolert enzyme-substrate method were 5.88%（4/68）and 35.29%（24/68）, respectively, with a significant difference （χ2=16.41，P<0.01）. The coincidence rate of the two methods was 64.71% (44/68), and the difference of the detection effects of the two methods was statistically significant (χ2=16.41，P=0.000). A total of 25 strains of Legionella pneumophila were isolated，and the serum types were mainly LP1（14/25）. Conclusion The Legiolert enzyme-substrate method represente a higher detection rate of Legionella pneumophila in water samples from public places than the conventional cultivation.
