Chemical Constituents of n-Butanol Extract Part of Akebia trifoliata Caulis
10.13422/j.cnki.syfjx.20192312
- VernacularTitle:三叶木通正丁醇部位化学成分分离鉴定
- Author:
Ming-ming YUAN
1
;
Xi WANG
1
;
Xi WU
1
;
Shun ZENG
1
;
Chuan-sheng ZOU
1
;
Rui-jian ZHONG
1
;
Guo-ping ZHOU
1
Author Information
1. Jiangxi Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China
- Publication Type:Research Article
- Keywords:
Akebiae Caulis;
Akebia trifoliata;
Akebia;
n-butanol extract
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(1):139-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To study the chemical constituents from n-butanol extract of Akebia trifoliata caulis. Method::The 100 kg caulis of A. trifoliata was extracted with 75% ethanol (EtOH) for three times by heating reflux. These 3 extracts were decompressed and concentrated, and then dissolved in water. The solvent was successively extracted with dichloromethane, ethyl acetate and n-butanol. The chemical constituents from the n-butanol fraction were isolated by macroporous, silica gel, sephadex LH-20 and ODS columns, and semi-preparative high performance liquid chromatography, and their chemical structures were determined through MS, NMR analysis (1H and 13C-NMR) and spectroscopic data from literatures. Result::Totally 14 compounds were isolated and identified as mutongsaponin B(1), mutongsaponin C(2), saponin PH(3), begoniifolide A(4), 2α, 3β, 23-trihydroxy-30-noroleana-12, 19-dien-28-oicacid-O-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(5), akemisaponins D(6), akemisaponins E(7), asiaticoside(8), saponin PJ1(9), scheffoleoside A(10), symplocosneolignan A(11), kalopanax-saponins D(12), leonticin E(13), ciwujianoside A1(14). Conclusion::Compounds 1-4, 11, 13, 14 were isolated from this plant for the first time. The discovery of these compounds further enriched the chemical constituents of A. trifoliata, and provided experimental and scientific basis for the comprehensive development and utilization of A. trifoliata.
