Investigation of Protective Effect of Different Bile and Their Arisaema cum Bile on Acute Lung Injury Rats Induced by LPS
10.13422/j.cnki.syfjx.20192153
- VernacularTitle:不同胆汁及其制成的胆南星对LPS诱导的急性肺损伤大鼠保护作用考察
- Author:
Ya-chen CUI
1
;
Li-qian SHAN
1
;
Xiao-feng LIU
1
;
Guo-shun SHAN
1
;
Hui GAO
1
Author Information
1. School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
- Publication Type:Research Article
- Keywords:
porcine bile;
oxgall;
sheep bile;
Arisaema cum Bile;
acute lung injury;
inflammatory factors;
lipopolysaccharide
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(1):125-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To compare the protective effect of different bile (porcine bile, oxgall and sheep bile) and their Arisaema cum Bile on rats with acute lung injury, so as to provide reference for the selection of bile and the classification of decoction pieces of Arisaema cum Bile. Method::Wistar male rats were randomly divided into 8 groups (n=12), including blank group, model group, porcine bile group, oxgall group, sheep bile group, Arisaema cum Bile with porcine bile group, Arisaema cum Bile with oxgall group and Arisaema cum Bile with sheep bile group. Rats in each treatment group were given corresponding drug solution by gavage at 2.52 g·kg-1 every day, and rats in the model group and the blank group were given the same volume of normal saline by gavage every day for a total of 8 days. On the 8th day, after 1 h of administration, rats in the model group and each treatment group were intraperitoneally injected lipopolysaccharide (LPS, 2 mg·kg-1) to prepare rat lung injury model. Blood and lung tissues were collected from every four rats at 3, 6, 24 h after LPS injection, respectively. Lung coefficient, lung water content and wet weight/dry weight ratio of lung tissue (W/D) were measured, and the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and thromboxane B2 (TXB2) in serum and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the contents of malondialdehyde (MDA) and matrix metalloproteinase-9 (MMP-9) in lung tissue were determined by enzyme linked immunosorbent assay (ELISA). The pathological morphology of rat lung tissue was observed and the score of lung tissue injury was calculated. Result::Compared with the model group at the same time point, the lung coefficient, W/D, lung water content, contents of TNF-α, IL-6 and TXB2 in serum, contents of MDA and MMP-9 in lung tissue of rats in each treatment group were all decreased, and most of them had significant differences (P<0.05, P<0.01), but the activities of GSH-Px and SOD were all increased, and most of them had significant differences (P<0.05, P<0.01). The oxgall group and the sheep bile group were superior to the porcine bile group in most of the indexes, the Arisaema cum Bile with oxgall group and the Arisaema cum Bile with sheep bile group were superior to the Arisaema cum Bile with porcine bile group, and some of them had significant differences(P<0.05, P<0.01). Conclusion::Each bile group and each Arisaema cum Bile group all have protective effects on rats with acute lung injury induced by LPS, and the therapeutic effect of oxgall and sheep bile is better than that of porcine bile, the therapeutic effect of Arisaema cum Bile prepared by oxgall and sheep bile is better than that of Arisaema cum Bile prepared by porcine bile.
